新品速遞丨6月上市的經(jīng)嚴(yán)格驗(yàn)證的全新抗體、試劑及試劑盒
STING (E9X7F) Rabbit mAb #90947
對(duì)使用 STING (E9X7F) Rabbit mAb（左圖，紅色）標(biāo)記以及使用 CD45R/B220 (RA3-6B2) Rat mAb (FITC Conjugate) #34399（右圖，綠色）和 DAPI #4083（右圖，藍(lán)色）共標(biāo)記的固定冷凍的小鼠淋巴結(jié)組織進(jìn)行共聚焦免疫熒光分析。
對(duì)使用 STING (E9X7F) Rabbit mAb（上圖，紅色）標(biāo)記以及使用 CD45R/B220 (RA3-6B2) Rat mAb (FITC Conjugate) #34399（下圖，綠色）和 DAPI #4083（下圖，藍(lán)色）共標(biāo)記的固定冷凍的小鼠脾臟組織在低放大倍率（左圖）和高放大倍率（右圖）進(jìn)行共聚焦免疫熒光分析。
對(duì)使用 STING (E9X7F) Rabbit mAb（左圖，紅色）標(biāo)記的固定冷凍的小鼠肺臟組織進(jìn)行共聚焦免疫熒光分析。然后將游離的二抗結(jié)合位點(diǎn)用 Rabbit (DA1E) mAb IgG XP? Isotype Control #3900 封閉，之后用 COL1A1 (E8F4L) XP? Rabbit mAb (Alexa Fluor? 647 Conjugate) #72827（右圖，偽彩青色）和 DAPI #4083（右圖，藍(lán)色）進(jìn)行共標(biāo)記。
使用 STING (E9X7F) Rabbit mAb（綠色）、β-Actin (8H10D10) Mouse mAb #3700（紅色）和 DAPI #4083（藍(lán)色）對(duì) J774A.1 細(xì)胞（左圖，高表達(dá)）和 MBT2 細(xì)胞（右圖，低表達(dá)）進(jìn)行共聚焦免疫熒光分析。
使用 STING (E9X7F) Rabbit mAb（綠色）、β-Actin (8H10D10) Mouse mAb #3700（紅色）和 DAPI #4083（藍(lán)色）對(duì) MOLP-8 細(xì)胞（左圖，陽性）和 A549 細(xì)胞（右圖，陰性）進(jìn)行共聚焦免疫熒光分析。
使用 STING (E9X7F) Rabbit mAb（實(shí)線）或濃度匹配的 Rabbit (DA1E) mAb IgG XP? Isotype Control #3900（虛線）對(duì) A549 細(xì)胞（藍(lán)色，陰性）和 THP-1 細(xì)胞（綠色，陽性）進(jìn)行流式細(xì)胞分析。Anti-rabbit IgG (H+L)、F(ab')2 Fragment (Alexa Fluor? 488 Conjugate) #4412 作為二抗。
使用 STING (E9X7F) Rabbit mAb（實(shí)線）或濃度匹配的 Rabbit (DA1E) mAb IgG XP? Isotype Control #3900（虛線）對(duì) NIH/3T3 細(xì)胞（藍(lán)色，低表達(dá)）和 J774A.1 細(xì)胞（綠色，高表達(dá)）進(jìn)行流式細(xì)胞分析。Anti-rabbit IgG (H+L)、F(ab')2 Fragment (Alexa Fluor? 488 Conjugate) #4412 作為二抗。
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干擾素基因刺激蛋白 (STING, TMEM173, MITA) 是一種跨膜接頭蛋白，它是細(xì)胞病原細(xì)胞漿 DNA 的天然免疫應(yīng)答的一個(gè)關(guān)鍵組分 【1,2】。STING 是一種被發(fā)現(xiàn)主要在內(nèi)質(zhì)網(wǎng)中普遍表達(dá)的蛋白 【1】。在細(xì)胞漿 DNA 的刺激下，酶 cGAMP 合成酶 (cGAS) 會(huì)產(chǎn)生第二信使環(huán)-GMP-AMP (cGAMP) 【3,4】。cGAMP 結(jié)合并激活 STING 【3,4】。此外，通過 DDX41 和 IFI16 等核酸感應(yīng)蛋白檢測(cè)細(xì)胞漿 DNA 會(huì)導(dǎo)致 STING 激活 【5,6】。激活后，STING 與 TBK1 轉(zhuǎn)位至核周內(nèi)體，并在 Ser366（小鼠內(nèi)為 Ser365）位點(diǎn)被 ULK1 磷酸化【7, 8】。TBK1 激酶磷酸化并激活 IRF-3 和 NF-κB，從而誘導(dǎo) I 型干擾素和其他免疫應(yīng)答基因 【1,2,7】。
關(guān)鍵詞：天然免疫，自身免疫病，腫瘤免疫
點(diǎn)擊查看更多詳情：STING (E9X7F) Rabbit mAb #90947
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貨號(hào) |
產(chǎn)品名稱 |
應(yīng)用 |
反應(yīng)性 |
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Adhesion / ECM |
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86903T |
WB, IP, IHC-Bond, IHC-P, IF-IC |
H |
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94386SF |
WB, IHC-P, IF-IC, F |
H, M, R |
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10962S |
WB |
H, M, (R) |
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97174SF |
WB, IHC-P, IF-IC, F |
H |
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68534S |
WB, IF-F, IF-IC |
H, M |
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20302T |
WB, IP, IHC-P |
H |
|
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Apoptosis |
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74860SF |
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (BSA and Azide Free) |
IHC-P, IF-IC, F |
H, M |
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95696SF |
Cleaved PARP (Asp214) (D64E10) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, Mk |
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Cell Cycle / Checkpoint Control |
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62548SF |
IHC-P, IF-IC, F |
H |
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60480SF |
WB, IHC-P |
H, Mk |
|
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46565SF |
WB, IHC-P, IF-IC, F |
H |
|
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41359SF |
Phospho-Rb (Ser807/811) (D20B12) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M, R, Mk |
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Chromatin Regulation / Nuclear Function |
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52626S |
WB |
H, M, R, Mk |
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73098S |
WB |
H, M, R, Mk |
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85449S |
WB |
H, R, Mk |
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87162S |
WB, IP, ChIP |
H, M, R, Mk |
|
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65992S |
PSPC1 (E7L8B) Rabbit mAb |
WB, IF-IC |
H, M, (R) |
|
Cytoskeletal Signaling |
|||
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62114S |
WB |
H, M, R |
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10333S |
WB |
H, M, R |
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56551S |
WB, IP |
H |
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46251S |
WB |
H, M, R |
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69319SF |
WB, IHC-P |
H, M, R |
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Developmental Biology |
|||
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72528S |
- |
- |
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87548S |
- |
- |
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87179T |
WB, ChIP |
H |
|
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60770T |
IHC-P, IF-IC |
H |
|
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42028S |
- |
- |
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25330S |
F |
H, M, R |
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81849S |
F |
H, M, R |
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50850S |
IHC-P |
M |
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Glucose / Energy Metabolism |
0 |
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40941S |
WB |
H, M, R |
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Immunology and Inflammation |
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47359SF |
WB, IHC-Bond, IHC-P |
M |
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32814S |
WB, IHC-Bond, IHC-P |
H |
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32814T |
WB, IHC-Bond, IHC-P |
H |
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53986C |
ELISA |
H |
|
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49392S |
WB, IP |
H |
|
|
87015S |
- |
- |
|
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87791SF |
Granzyme B (E5V2L) Rabbit mAb (Mouse Specific) (BSA and Azide Free) |
WB, IHC-Bond, IHC-P, F |
M |
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29881S |
WB, IP, IF-IC |
H |
|
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37517S |
WB, IP, IHC-P |
H |
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50384S |
IHC-Bond, IHC-P |
H |
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33469S |
IHC-Bond, IHC-P |
H |
|
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54059S |
- |
- |
|
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95742SF |
WB, IHC-Bond, IHC-P, IF-F, F |
M |
|
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98018S |
- |
- |
|
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64063S |
- |
- |
|
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79627S |
- |
- |
|
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84087S |
- |
- |
|
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74906S |
WB |
H |
|
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60847SF |
IHC-Bond, IHC-P |
H |
|
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39356SF |
WB, IHC-P |
H |
|
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24224SF |
PD-L1 (Extracellular Domain Specific) (E1J2J?) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P |
H |
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37908S |
WB, IHC-P |
H |
|
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37908T |
WB, IHC-P |
H |
|
|
58551S |
WB, F |
H |
|
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88845SF |
Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M |
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73533SF |
Phospho-Stat3 (Tyr705) (D3A7) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-Bond, IHC-P, IF-IC, F |
H, M, R, Mk |
|
51879SF |
Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, F |
H, M |
|
90947S |
IF-F, IF-IC, F |
H, M |
|
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16769S |
- |
- |
|
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24095S |
- |
- |
|
|
87804S |
WB |
H |
|
|
Infectious Diseases |
|||
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19595S |
WB |
Vir |
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93220S |
WB |
Vir |
|
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MAP Kinase Signaling |
|||
|
77955SF |
Phospho-c-Jun (Ser73) (D47G9) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-F, IF-IC, F |
H, M, R, Mk, Pg |
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62979SF |
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M, R, Mk, Mi, Pg, Sc |
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45899SF |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M, R, Hm, Mk, Mi, Dm, Z, B, Dg, Pg, Sc |
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55773SF |
Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC |
H, Mk |
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58328SF |
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P |
H, M, R, Dm, Sc |
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Metabolism |
|||
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63536S |
WB |
H, M, R |
|
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49381S |
WB |
H, M, R |
|
|
84476S |
WB, IP |
H |
|
|
Motif Antibodies |
|||
|
68517S |
- |
- |
|
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Neuroscience |
|||
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39561SF |
Phospho-CREB (Ser133) (87G3) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-F, IF-IC, F |
H, M, R |
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58595S |
WB, IF-F, IF-IC |
H, M, R |
|
|
57187S |
WB |
M |
|
|
PI3K / Akt Signaling |
|||
|
80722SF |
Phospho-Akt (Thr308) (D25E6) XP? Rabbit mAb (BSA and Azide Free) |
WB, IF-IC, F |
H, M, R, Mk |
|
54932SF |
Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific) (BSA and Azide Free) |
IHC-P |
H |
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Protein Folding and Trafficking |
|||
|
72312S |
WB |
H, M, R |
|
|
RTK |
|||
|
64191SF |
Phospho-CSF-1R/M-CSF-R (Tyr723) (49C10) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P |
H, M |
|
Translational Control |
|||
|
39788SF |
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M, R, Mk, Dm |
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16300S |
WB, ChIP |
H, M, R, Mk |
|
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49253S |
WB, IP |
H, M, R, Mk |
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88145S |
WB |
H, M, R, Mk |
|
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41645S |
WB |
H |
|
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36103S |
WB, IP, IF-F, IF-IC |
H, M, R, Mk |
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59753S |
WB, IP |
H, M, R, Mk |
|
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81736SF |
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP? Rabbit mAb (BSA and Azide Free) |
WB, IHC-P, IF-IC, F |
H, M, R, Mk, Mi, Sc |
|
50837S |
WB, IP |
H |
|
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Related Products |
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10612SF |
WB, IHC-P, IF-IC, F |
All |
|
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60395S |
Mouse Progenitor Exhausted CD8+ T Cell Markers Flow Cytometry Panel |
F |
M |
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【參考文獻(xiàn)】
1.Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
2. Zhong, B. et al. (2008) Immunity 29, 538-50.
3. Sun, L. et al. (2013) Science 339, 786-91.
4. Wu, J. et al. (2013) Science 339, 826-30
5. Zhang, Z. et al. (2011) Nat Immunol 12, 959-65.
6. Unterholzner, L. et al. (2010) Nat Immunol 11, 997-1004.
7. Ishikawa, H. et al. (2009) Nature 461, 788-92.
8. Konno, H. et al. (2013) Cell 155, 688-98.
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